BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube, Each. BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube. Remarks: Glucose – Any degree of yellow is positive. Acid end products from glucose fermentation turn the pH indicator from red (alkaline) to yellow (acid). of a Multitest System (Enterotube) for Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification . (ii) Modified lysine-lactose me- dium: a .. Bergey’s manual of determinative bacteriology.

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Pathogens virulence factors Yersinia pestis: After completing this project the students should be able to: Name 10 routine chemical tests performed on urine and list a More information. To make this website work, we log user data and share it with processors. Explanation of the Test. Information on the appearance of positive and negative reactions is provided in Table 1.

They are catalase positive and nearly all of them are oxidase negative [It has been proposed recently to include Plesiomonas shigelloides into the Enterobacteriaceae based on 16S rrna sequencing and because it contains the enterobacterial common antigen. Hydrogen sulfide is produced by light amber bacteria capable of reducing sulfur-containing compounds, such as peptones and sodium thiosulfate present in the medium.

BBL Enterotube II may be used to identify aerobic, facultatively anaerobic Gram negative rods Enterobacteriaceae isolated from any specimen. Examples of oxidase-negative non-enterobacteriaceae that fail to ferment glucose under anaerobic conditions in BBL Enterotube II are glucose negative Acinetobacter species. Elsevier Science Publishing Co. Add drops of Kovacs reagent cat. Amyl or Isoamyl Alcohol 75 ml p-dimethylaminobenzaldehyde 5 g Hydrochloric acid, concentrated 25 ml Dissolve p-dimethylaminobenzaldehyde the reagent should ejterotube light yellow in color in alcohol and then slowly add the acid.

Biochemical identification of bacteria Dr. As we shall explore in this lab exercise.

Enterotube ii computer coding manual – Google Docs

Certain microorganisms will not always produce the ideal strong positive color change. Some Providencia strains may produce a diffuse brown coloration in this medium, however, this should not be confused with true H 2S production as indicated by the presence of black color. Selective media allow certain types of organisms to grow, and inhibit the growth. Biochemical identification of bacteria. We proposed in the Basic Microbiology More information.


Madi, and Mohamed H. With the exception of indole and VP, read the reactions in a sequential fashion by comparing the colors of the media in the tube after incubation with those given in the color scheme on the cover of the coding pad and eventually with an uninoculated BBL Enterotube II which must be brought to room temperature first Figure 5.

Once we have obtained any growth, work flow in the Microbiology. Add circled numbers in the bracketed section and enter the sum in the space provided below the arrow Figure 6. The enclosed inoculating wire allows inoculation of all compartments in one step from one or a few single colonies of an isolate. Bacterial decarboxylation of lysine, which results in the formation of the alkaline end product cadaverine, is indicated by a change in the color of the indicator in the medium from pale yellow acidic to purple alkaline.

Bacterial fermentation of dulcitol, which results in the formation of acidic end products, is indicated by a change in color of the indicator present in the medium from green alkaline to yellow or pale yellow acidic. The portion of the wire remaining in the tube maintains anaerobic conditions necessary for true fermentation of glucose, production of as and decarboxylation of lysine and ornithine. The bacteria thus isolated needs to be further identified to genus More information.

Name 10 routine chemical tests performed on urine and list a. Urine Culture Melissa Schreiber, Presenter: Able to detoxify toxic by products of oxygen. Decarboxylation of ornithine, which results in the formation of the alkaline end product putrescine, is indicated by a change in the color of the indicator in the medium from pale yellow acidic to purple alkaline.



In the example given in Figure 6, the identification as Klebsiella pneumoniae is obtained. Gelatin Hydrolysis Test Protocol The Gelatin Hydrolysis Test is used to detect the ability of microorganisms to produce the enzyme gelatinase.

Voges-Proskauer reagents may be prepared as follows: For details, consult the references While a huge variety of genera and species has been described over the years, the organisms that are isolated from clinical specimens belong to 20 to 25 species that are well-known for many years. The Chemistry of Carbohydrates Experiment 5 Objective: Ancillary culture media, reagents and laboratory equipment as described.

The presence of acetoin is indicated by the development of a red color within 5 to 20 min. Depending on this decision, use the frontside or the backside of the coding pad. Media, incubation, and aseptic technique Lab Exercise 3: All organisms require a supply of energy and matter to build themselves and to continue to function.

Use isolates from appropriate media see Test Procedure. Identification of Gram negative bacteria should be made with the consideration of additional characteristics such as source of specimen, history of the patient, colonial and microscopic morphology, serology and antimicrobial susceptibility patterns.


All reactions which should be positive may be of equal, greater or lesser intensity than the colors indicated in the Color Reaction Chart. The tubes may be inoculated up to the expiration date and incubated for the recommended incubation times.

Principle Urine cultures are performed to detect. The medium is covered with wax to provide anaerobic conditions and to allow detection of gas formation. Properties of Proteins Experiment 8 Objective: