COUNTER CURRENT IMMUNOELECTROPHORESIS PDF

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AIM: To learn the technique of Counter Current Immunoelectrophoresis. PRINCIPLE: Immunoelectrophoresis is the electrophoresis of a determined antigen. A counter current electrophoresis technique for the rapid detection of Mycoplasma mycoides subsp, Mycoides antigen in tissue from animals infected with. Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an See also[edit]. Immunoelectrophoresis · Electrophoresis protein · Procalcitonin · Total complement activity · MELISA · Complete blood count · lymphocyte count.

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Observations on the carriage of pneumococci by patients with chronic bronchitis, and their families. Retrieved from ” https: National Center currnet Biotechnology InformationU. The optimum conditions for performing the test are given. Changes in the bacterial flora of the sputum associated with exacerbations and long-term antibacterial treatment.

Diagnostic immunology Nephelometry Complement fixation test Immunocytochemistry Immunohistochemistry Direct fluorescent antibody Epitope mapping Skin allergy test Patch test. Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis.

Correlation of circulating capsular polysaccharide with bacteremia in pneumococcal pneumonia. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a precipitin line, indicating binding.

This page was last edited on 3 Septemberat Vurrent can help Wikipedia by expanding it. Associated Data Supplementary Materials. Journal List J Clin Pathol v. M El-Refaie and C Dulake. Support Center Support Center. Pneumococcal typing and the problem of endogenous or exogenous reinfection in chronic bronchitis. Group-specific Neisseria meningitidis antigens present in the serum of patients with fulminant meningococcemia.

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An evaluation of sputum examination in chronic bronchitis. Value of dilute inocula in cultural examination of sputum.

Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.

Immunologic tests Blood tests Immunology stubs. Bacteriologic flora of the lower respiratory tract.

J Lab Clin Med. Etiologic diagnosis of pneumococcal pneumonia. Br J Dis Chest. Abstract Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum.

Transtracheal aspiration in the counher of patients with pneumonia. From Wikipedia, the free encyclopedia. Better precipitin lines are obtained when the gel-support is acid pH than when it is alkaline pH The chemotherapy of bacterial respiratory infections. Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigenit is similar to immunodiffusionbut with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel.

Medical tests used in immunology and for inflammation CPT — The clinical importance of these findings is still being studied but our experience has shown that patients with chest infections should have effective antipneumococcal antibiotics as part of their regimen currrent that the laboratory diagnosis may be made quickly and accurately by counter-current immunoelectrophoresis.

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Omniserum is as effective as group-specific sera for the identification of pneumococcal capsular antigens. Am Rev Respir Dis.

Nosocomial respiratory infections with gram-negative bacilli. N Engl J Med. This immunology article is a stub. By using this site, you agree to the Terms of Use and Privacy Policy. This article has been cited by other articles in PMC.

Open in a separate window. Please review our privacy policy. Effect of previous antimicrobial therapy on bacteriological findings in patients with primary pneumonia. The John Barnwell Lecture. Views Read Edit View history.

The significance of colonization of the respiratory tract. Immunoprecipitation Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis.

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J Hyg Lond Jun; 53 2: Detection by immunoelectrophoresis of antigen in sera of patients with pneumococcal bacteraemia. Types of pneumococci found in blood, spinal fluid and pleural exudate during a period of 15 years Pneumococcal antigen in lobar pneumonia.

Acta Pathol Microbiol Scand. Non-tuberculous bacterial flora of sputum and of the upper and lower respiratory tract in pulmonary tuberculosis. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis.